Differential utilisation of ceramide during replication of the flaviviruses West Nile and dengue virus

TE Aktepe; H Pham; JM Mackenzie

Journal article

Differential utilisation of ceramide during replication of the flaviviruses West Nile and dengue virus

TE Aktepe, H Pham, JM Mackenzie

Virology | Published : 2015

DOI: 10.1016/j.virol.2015.06.015

Abstract

It is well established that +ssRNA viruses manipulate cellular lipid homoeostasis and distribution to facilitate efficient replication. Here, we show that the cellular lipid ceramide is redistributed to the West Nile virus strain Kunjin virus (WNVKUN) replication complex (RC) but not to the dengue virus serotype 2 strain New Guinea C (DENVNGC) RC. We show that prolonged chemical inhibition of serine palmitoyltransferase with myriocin had a significant deleterious effect on WNVKUN replication but enhanced DENVNGC replication. Additionally, inhibition of ceramide synthase with Fumonisin B1 had a detrimental effect on WNVKUN replication and release of infectious virus particles but contrastingl..

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University of Melbourne Researchers

Turgut Aktepe Author

Jason Mackenzie Author

Related Projects (1)

LIPIDS STRESS AND FLAVIVIRUS REPLICATION

What happens when viruses infect cells? How do they control the cells they infect? How do the make the cells do the things the virus wants? ..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

We thank Alexander Khromykh, Roy Hall and Paul Young for generously providing the WNV and DENV antibodies, and David Jans for providing DENV<INF>NGC</INF>. We also thank Daniel Watterson (University of Queensland) for help and advice with the DENV foci-forming assay. This research was supported by a Project grant (No. 1004619) to J.M. from the National Health and Medical Research Council of Australia.